Carbohydrazides functionalized cellulose (CH-MC). was prepared and characterized using some qualified techniques such as Scanning Electron Microscopy (SEM), Infra-Red (FT-IR), Elemental analysis (EA). The prepared CH-MC was employed for uptake of heavy metal ions such as, Hg²⁺ from different samples. Sorption parameters: such as time of sorption, pH, temperature and the concentration of sorbent were investigated to determine the best conditions for sorption. The kinetic of sorption agreed with the second-order model and the chemical adsorption is the rate-limiting step. In addition, the sorption isotherm experiments revealed that the best adequate with Langmuir model sorption uptake of (CH-MC) were 64 mg. g^-1 for Hg²⁺. The real samples including Hg²⁺ were used for analytical applications on the present methodology and the observed data is promising