Renal injury (RI) is one of the most main causes of disability and mortality in several countries in the world. RI is caused when the kidneys are damaged and not functioning as they should. Renin-angiotensin-aldosterone system's (RAAS) overactivity, promotes vasoconstriction, hypertension, and renal inflammation. It is linked to the advancement of kidney disease. Herein, we assessed the ameliorative effect of treatment with beta carotene-loaded silica nanoparticles (βC-SiNPs) as a reno-protective agent on renal function and structure using N^ω-nitro-L-arginine methyl ester (L-NAME) for induction renal injury in the male rats. Thirty-six rats were divided into six groups: Normal group (N); native beta carotene (N+βC) group; βC-loaded silica nanoparticles (N+βC-SiNPs) group; L-NAME group; L-NAME +βC group and L-NAME + βC-SiNPs group. After L-NAME and/or βC-SiNPs were administrated orally to male rats for 4 weeks, blood samples and kidneys were taken for biochemical analyses and histopathological studies. The obtained results demonstrated that the sol-gel synthesized SiNPs show spherical nanoparticles with average particles size of 290 ± 120 nm. The βc- SiNPs particles also exhibited spherical morphology with average particles size of 460 ± 160nm. βC-SiNPs have a significant reversal effect on kidney function, and markedly improved renal histopathological markers of inflammation and fibrosis.