A polymeric thin mixture of polyvinyl chloride (PVC)/ polyvinylidene fluoride (PVDF) (30%:70% wt) blend dopant with gradient concentrations of synthesized silver nanoparticles was prepared. Silver nanoparticles were gained from the laser ablation process. Nanocomposite blends were investigated versus gram-positive (Staphylococcus aureus, Bacillus subtilis) in addition to negative grams (Escherichia coli, Pseudomonas aeruginosa). Bacterial cells were handled with the nanocomposite blend, and the growth rates were investigated beneath varying the concentrations of Ag-NPs, incubation times, incubation temperatures plus ph.  It was obvious that the increase in the nanoparticle content was combined with an increase in the activity index of the corresponding prepared composite samples. The manufactured PVC/PVDF/Ag-NPs blend could be proposed for improving the biological properties, wound healing, and dressing applications.