Aim: To investigate the exact role of vitamin D3, CD68 and CD1-alpha in non-muscle invasive bladder cancer (NMIBC).  Subjects and Methods: This study including ( 99)  male with non-muscle invasive bladder cancer NMIBC (Ta-T1) who were subjected to transurethral resection (TURBT). As well as control group consists of 50 male individuals. We determined the gene expression level for CD68, CD1-alpha and the protein concentration of vitamin D3 level in blood samples in patients and controls.  Results: Compared to the control group, vitamin D3 levels were considerably lower in the study group. There was no significant correlation between vitamin D3 with either grade or stage. In comparing the recurrence and non-recurrence groups, vitamin D was significantly increased in non-recurrence group. CD68 was highly expressed in recurrence more than nonrecurrence and control group patient but in case of CD1-alpha gene expression; it was significantly decrease in case of recurrence in a comparison to nonrecurrence and control patients. Conclusions: The results suggested the possible effect of vitamin D3, CD68 and CD1-alpha in reducing the recurrence status, as presented, CD1-alpha and vitamin D3 were significantly increased in non-recurrence cases; but CD68 was significantly decrease in non- recurrence.