Enuresis is the most prevalent urologic complaint in paediatric patients and is defined as the involuntary leakage of urine during sleep that happens at least twice per week in children older than 5 years old (or the developmental equivalent) for at least 3 months. The aims of the study were to assess the level of serum MMP-9 and Malondialdehyde (MDA) in children with nocturnal enuresis and to look for a relation between level of metalloproteases and the risk of nocturnal enuresis in adolescence.  Methods: 53 patients (10-18 years old) and 47 healthy children were included in the study. Serum MMP-9 were measured in control and cases groups. Results: Nonsignificant difference between studied groups in MMP-9 levels in patient  group compared to control group. Conclusions, Nocturnal enuresis were found non difference between studied groups in MMP-9 and MDA levels in cases group compared to control group.  More studies are recommended to understand the development of the disease and its prevention.