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413183

Identification of Lactobacillus Species from Vaginal Secretions of Healthy and Women with Vaginitis by Molecular Methods

Article

Last updated: 25 Feb 2025

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Subjects

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Tags

Clinical microbiology

Abstract

Background: The Lactobacillus species bear a high responsibility toward vaginal microbiota and, accordingly, play a very important role in the maintenance of vaginal health. In healthy women, Lactobacillus bacteria keep the vagina in an acid environment and helps in maintaining the microbiota balance. Objective: To identify and compare Lactobacillus species in vaginal secretions of healthy women and those with vaginitis, by using molecular techniques. Methodology: The study was conducted in College of Medicine laboratories- University of Anbar and Private Specialized laboratories from November 2023 until October-2024. 234 specimens were collected from women with expected vaginitis, their ages ranged from 20-50 years they were selected from Al-Ramadi Teaching Hospital for maternity and pediatrics in Ramadi, Western Iraq. Genomic DNA extraction from vaginal secretions was performed using Presto™ Mini gDNA Bacteria Kit. The Nano drop instrument was used to calculate DNA purity and concentration. Multiplex Polymerase chain reaction (PCR) was carried out using Multiplex-PCR primer design for ten of Lactobacillus species. All Multiplex-PCR reactions were done in Applied Bio-system 2720 thermo cyclers, and amplification of target gene. Results: The results of sampling show that 148/234 of specimens were identified a vaginitis positive while 86/234 were negative. The molecular screening findings by Multiplex-PCR indicated that five species were identified as L. jensenii, L. acidophilusL. crispatus,L. iners, L. gasseri as common species in the vaginal microbiome. Conclusion: The analysis results show that the more frequent species in women case was L. crispatus comparing to the control group with p-value was 0.001, they were present in 26 samples of patients versus 11 samples of controls. The consistent presence of Lactobacillus iners across different types of vaginitis is a notable finding

DOI

10.21608/ejmm.2025.354413.1446

Keywords

Keywords: Lactobacillus, Microbiome, Vaginal secretions, Multiplex Polymerase Chain Reaction, Vaginitis

Authors

First Name

Deena

Last Name

Kamil

MiddleName

A.

Affiliation

Microbiology department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

din22m0004@uoanbar.edu.iq

City

-

Orcid

-

First Name

Muntaha

Last Name

Al-Alouci

MiddleName

M.

Affiliation

Microbiology department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

-

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-19

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_413183.html

Detail API

http://journals.ekb.eg?_action=service&article_code=413183

Order

413,183

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Identification of Lactobacillus Species from Vaginal Secretions of Healthy and Women with Vaginitis by Molecular Methods

Details

Type

Article

Created At

25 Feb 2025