To assess the response of three sugar beet varieties to different levels of humic acid fertilizer on growth, yield, and quality of sugar beet, two field experiments were carried out at a private farm in El-Fayoum Governorate (latitude 29. 19° N and longitude 30. 49° E) during the 2022/2023 and 2023/2024 seasons. The study included nine treatments, which represented the combinations of three mono-germ sugar beet varieties (Nazarea, Jamajka, and Bts7715) and three levels of humic acid added as soil amendment (without, 4 and 8 liters/400 liters of water/fed). A randomized complete block design using a split-plot distribution with three replications was used. Results revealed that applying 8 L humic acid/fed appreciably increased root criteria, sucrose%, root, and sugar yields/fed while the sugar lost to molasses (SLM) % decreased over the two seasons and improved alkalinity coefficient value in the 2^nd season, compared to those received 4 L humic acid. However, fertilizing beets with 4 or 8 L humic/fed (without a significant difference) gave the highest values of extracted sugar %, quality index, and foliage yield/fed compared to check treatment over the two seasons. The Nazarea variety outperformed the others by producing the thickest, heaviest roots and foliage fresh weight/plant. It also had the highest root yield/fed and alkalinity coefficient value, as well as the lowest SLM in the two growing seasons. while the Nazarea and Bts 7715 varieties achieved the highest foliage and sugar yields/fed (without a significant difference) in either season. Both Bts 7715 and Jamajka varieties (with no significant difference) displayed the highest sucrose and extracted sugar percentages with the lowest sodium content, resulting in an improved quality index.