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412092

Efficacy and biochemical analysis of sub-Lethal concentrations of some insecticides against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Article

Last updated: 25 Feb 2025

OverviewSimilar Items

Subjects

-

Tags

Plant pest management

Abstract

The study assessed the toxicity and biochemical effects of emamectin benzoate, lufenuron, chlorpyrifos, and spinosad on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) 2nd and 4th instar larvae. Results revealed that, 2nd instar larvae were more sensitive than 4th instar larvae to the four insecticides. Emamectin benzoate exhibited the highest toxicity (LC50: 0.166 ppm and 0.23 ppm), followed by lufenuron, chlorpyrifos, and spinosad. Biochemical analysis revealed significant metabolic disruptions. Total soluble protein levels increased after emamectin and spinosad treatments but decreased with lufenuron. Carbohydrate levels rise with emamectin and lufenuron but dropped with chlorpyrifos and spinosad, while lipid content decreased across all treatments, indicating high energy demands. Glutathione S-transferase (GST) activity increased substantially, reflecting enhanced detoxification processes, particularly with emamectin. Alpha and beta esterase activities also increased, further supporting detoxification mechanisms. Alkaline phosphatase activity showed pronounced increases, especially with emamectin, while acid phosphatase levels declined, suggesting disrupted lysosomal activity. Acetylcholinesterase (AChE) activity decreased in all treatments, with chlorpyrifos causing the greatest inhibition. Significant increases in GOT/AST and GPT/ALT activities indicated cellular damage and metabolic stress, with chlorpyrifos showing the strongest effect, followed by emamectin. These findings highlight the varying toxicity levels and metabolic disruptions caused by the tested insecticides, providing valuable insights for supporting management strategy of S. frugiperda.

DOI

10.21608/asejaiqjsae.2025.412092

Keywords

Spodoptera frugiperda, Emamectin benzoate, lufenuron, chlorpyrifos, and spinosad, ALP, GST, AChE, ACP, Biochemical studies

Authors

First Name

Belal

Last Name

Soliman

MiddleName

Soliman Mohammed

Affiliation

Department of Plant Protection, Faculty of Agriculture, Damanhour University, Egypt

Email

bsoliman91@gmail.com

City

Beheira Governorate

Orcid

-

Volume

46

Article Issue

1

Related Issue

52830

Issue Date

2025-03-01

Receive Date

2025-01-10

Publish Date

2025-03-31

Page Start

81

Page End

90

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 46_Issue 1_Pages 81-90.pdf

PDF . 318.4kB

Link

https://asejaiqjsae.journals.ekb.eg/article_412092.html

Detail API

http://journals.ekb.eg?_action=service&article_code=412092

Order

6

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Efficacy and biochemical analysis of sub-Lethal concentrations of some insecticides against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Details

Type

Article

Created At

25 Feb 2025