Background: Helicobacter pylori can be an etiology of chronic gastritis, peptic ulcer, and stomach cancer. The aim was to evaluate the relationship between H. pylori infection and alterations in some hematological and biochemical parameters among the Egyptian people. Method: Ninety individuals with confirmed H. pylori infection by the fecal antigen were selected and then followed after a month of treatment with Levofloxacin, amoxicillin, and a proton pump inhibitor; they underwent testing again for hematological and biochemical parameters after treatment of H.pylori. Results: both groups, group A (Before treatment) and Group B (After 1 month of therapy), showed a statistically significant relation between the presence of H. pylori and the CBC values; patients with pre-existing dyslipidemia exhibited a link between H. pylori infection and reduced levels of HDL-C as well as elevated LDL-C and triglycerides. Conclusion: H. pylori is associated with changes in some hematological and biochemical parameters.