The study herein explores the possible uses of plant extracts obtained from common milkweed, Egyptian henbane, neem, sweet clover, datura, and ashwagandha, which were applied at concentrations of 0.5%, 1%, 2%, and 4% to control the two-spotted spider mite, T. urticae. The laboratory experiments assessed the mortality rates of adult females exposed to different concentrations of acetone and methanol extracts were calculated in vitro. Datura, ashwagandha, neem, and sweet clover extracts performed better when extracted with methanol solvent than with acetone at a concentration of 4%. These extracts caused mortality rates of 85.71, 83.52, 75.82, and 46.15 % for methanol solvent and 84.62, 78.02, 69.23, and 13.18 % for acetone solvent, respectively. These results were further validated in field experiments on eggplant crops, where ashwagandha extracted by Methanol solvent was a potent extract against T. urticae when applied at 4% concentration, reducing the pest population by 68.06, 60.58, 75.29, 54.80, and 68.66 % after 1, 3, 7, 14, and 21 days, respectively, with an average of 65.47%. Datura extract reduced the pest population by 59.34%, 63.14%, 72.03%, 78.75%, and 62.77% after the above-mentioned periods, with an average of 67.20%. While neem and Egyptian henbane extracts gave similar reduction rates of 54.32, 58.71, 62.44, 54.19 and 62.87% for neem and 45.58, 53.16, 61.11, 67.86 and 52.77 % for Egyptian henbane, with an average of 58.50% and 56.09 % for neem and henbane, respectively. The observed efficacy among plant extracts emphasizes the importance of considering laboratory and field conditions for pest management strategies in agriculture.