Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract and is divided into Crohn's disease and ulcerative colitis. Objective: This study aimed to find the correlation between vitamin D deficiency and IBD activity in pediatric patients. Patients and Methods: A case-control study was conducted at the Gastroenterology Clinic, Pediatric Department, Zagazig University Hospital performed on 36 subjects divided into two equal groups; (group A) was a comprehensive sample, and (group B) contained apparently healthy participants as a control group of the same age, sex, and ethnically matched to the cases in the period between March 2021 to September 2021. Results: The Mean 1,25 dihydroxycholecalciferol (ng/l) levels in the remission and active phases were 28.18 ± 3.42 and 14.06 ± 3.92 respectively and the fecal calprotectin ranged from 50 to 257 with a mean of 118.28 ± 55.06. There was a non-statically significant correlation between Vit D and  1,25 dihydroxycholecalciferol levels in the remission and active phases. Conclusion: This study found that 1, 25 dihydroxycholecalciferol level was lower in patients with IBD than in healthy people.