IN ORDER to evaluate the oxidative defense and growth of grain sorghum [Sorghum bicolor (L.) Moench] grown under salt stress (2500 and 5000 ppm than the tap water as a control) to application of alpha-tocopherol and thiamine (as antioxidants) a pot experiment was conduct in the greenhouse of the National Research Centre, Dokki, Cairo, Egypt. The obtained results showed that salinity decreased the dry weight of root, stem and whole plant and root more affected than stem and whole plant but leaves seemed to be without effect. On reverse, T/R ratio (top to root ratio) increased as the salinity level increased. This means that salinity affect the root more than any part of grain sorghum plants. It is clearly shown that different grain sorghum plants parts dry weight and their whole weight increased by tocopherol or tocopherol+thiamine (spraying on vegetative parts) in comparable with the control plants. On the opposite side, T/R decreased with tocopherol+thiamine only. This means that this treatment increased dry weight of root than the other part of sorghum plant. It is clearly shown from data that different grain sorghum plants parts dry weight increased by tocopherol or tocopherol+thiamine spraying in comparable with the control plants. On the opposite side, T/R decreased with tocopherol+thiamine only. This means that this treatment increased dry weight of root than the other part of sorghum plant. O2- and MDA (malondialdhiede) its concentrations negatively responded to alpha-tocopherol or alpha-tocopherol+thiamine. The depression in these parameters by alpha-tocopherol were: 52.07 and 30.08 and by alpha-tocopherol+thiamine were 59.50, and 38.52%, respectively compared to that of the control. On the other side, APX (ascorbate peroxidases), GR (glutathione reductase), PPO (polyphenole oxidases) and CAT (catalase) level increased by: 83.81, 53.50,61.88 and 19.64 % as the grain sorghum plants sprayed by alpha-tocopherol and thiamine, respectively it is compare to the plants received distilled water. These Data lead to conclusion that application of alpha-tocopherol or thiamine increased tolerance of grain sorghum plants.