Background: Urinary tract infections are the fifth most common type of health care-associated infection. Up to 70%-80% of these infections are due to urethral catheterization. Catheter associated urinary tract infections account for increased morbidity and medical expenses. Aim of the study: This study aimed to assess the effect of betadine versus normal saline on urinary tract infection among patients with indwelling urinary catheter. Design: A Quasi experimental research design was utilized to achieve the aim of this study. Setting: The study was conducted at Intensive Care Unit of Assuit police Hospital affiliated to Ministry of Inferio. Study subjects: A Purposive sample of 70 female patients with indwelling urinary catheter in the previous mentioned setting with no any other genito-urinary disorders at the time of admission and with no immune compromised status. Data collection tools: Tool I: Patient Assessment Tool. Tool II: Urinary Tract Infection Clinical Symptoms Assessment. Tool III: Urine Microbiological Analysis. Results: The study showed that, the mean age of the studied patients was 37.76±10.9 years, 74,3% of the studied patients in betadine group had positive growth of microorganism in urine culture and 82,9% of the studied patients in normal saline group had positive growth of microorganism in urine culture. Conclusion: The study findings concluded that, there was no significant statistical difference between betadine and normal saline in relation to urinary tract infection among patients with indwelling urinary catheter. Recommendation: A similar study should be replicated on a large sample and other place to generalize the finding.