The present study was designed to investigate the chemical composition of thyme and basil medicinal plants, studying and evaluating the antioxidant and antimicrobial activity of oils, the oils under study were thyme (Thymus vulgaris L.) belonging to the family Lamiaceae and basil (Ocimum basilicum) belong to family Lamiaceae too.  The major compounds found in thyme essential oil, were thymol and cymene, while the major compounds found in basil essential oil, were linalool and 1,8-cineole. The antimicrobial activity of the investigated essential oils was tested against eight bacterial (two gram +ve bacteria and six gram -ve bacteria) and four fungal strains. Which were (S. aureus and enterococcus sp.) for gram positive, (E. Coli, S. typhi, acinitobacter sp., prouteus vulgaris, prouteus mirabillis and klebsiella pneumonaie) for gram negative, four fungal (candida albicans, rhizobium sp., fusarium sp < /em>. and alternaria A.). The quenching of DPPH free radicals and ferric-reducing antioxidant power assay further highlighted the antioxidant potential of thyme and basil essential oils.