Beta
ManageChatDiscover
411562

Purification and kinetics of uricase from fenugreek

Article

Last updated: 15 Feb 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Uricase (EC 1.7.3.3) is an enzyme involved in the purine breakdown pathway. The enzyme was isolated from fenugreek (Trigonella foenum-graecum) leaves and purified by 80% ammonium sulfate, DEAE-cellulose and Sephadex G-200. The final specific activity was 230.76 Umg-1 protein and the final yield of purification was 2.87 % with 133.38-fold. The optimal pH and the optimal temperature were 8.0 and 40°C. The enzyme was inactivated by the chelating agent as α-α-dipyridyl and the  IC50 value of α-α-dipyridyl was 9.03 mM.

DOI

10.21608/mjcc.2023.411562

Keywords

Uricase, Fenugreek (Trigonella foenum-graecum L.), Purification, Characterization

Volume

62

Article Issue

1

Related Issue

53780

Issue Date

2023-08-01

Receive Date

2025-02-13

Publish Date

2023-08-01

Page Start

56

Page End

61

Print ISSN

1687-5060

Online ISSN

2974-4938

Article File

MJCC_Volume 62_Issue 1_Pages 56-61.pdf

PDF . 1.4MB

Link

https://micc.journals.ekb.eg/article_411562.html

Detail API

http://journals.ekb.eg?_action=service&article_code=411562

Order

411,562

Type

Original Article

Type Code

3,440

Publication Type

Journal

Publication Title

Mansoura Journal of Chemistry

Publication Link

https://micc.journals.ekb.eg/

MainTitle

Purification and kinetics of uricase from fenugreek

Details

Type

Article

Created At

15 Feb 2025