Extract, describe the properties of a thermophilic fungal amylase, Aspergillus fumigatus which isolated from different soil samples and explore its antimicrobial activity. Six distinct fungal strains were isolated from Mansoura University soil samples using standard media, examined for characteristics, and tested for amylase production using a modified Czapek-Dox medium supplemented with starch. Results: Using Czapek's agar media, the isolated fungi were screened for the synthesis of amylase. One fungus strain was chosen and identified as Aspergillus sp. based on the pink color zone that developed. The most active species was Aspergillus fumigatus, which was identified from soil samples. After 30 minutes at 50 °C and pH 7.0, the organism that had been cultivated under submerged fermentation conditions generated amylase. In conclusion, Since Aspergillus fumigatus was isolated from soil samples demonstrating substantial amylase activity, it can be further used for the commercial manufacture of amylase. Amylase is an antibacterial agent that works well