Abstract Background: Telomeres are specialized chromatin struc-tures found at the ends of eukaryotic chromosomes; their length is an important factor in the early stages of embryonic develop-ment in humans. While studies have shown that different sperm preparation methods affect concentration, motility, and mor-phology, little is known about the probable association between telomere length and sperm preparation methods. Aim of Study: The purpose of this research was to exam-ine the comparison of a raw sample with a sample prepared by gradient. Material and Methods: Thirty semen samples were ob-tained from thirty patients for this prospective cohort study. From January 2021 to October 2021, participants admitted to the Obstetrics and Gynecology Department, in vitro fertiliza-tion (IVF) unit, Faculty of Medicine, Cairo University, were evaluated. Following clearance from Cairo University's Local Ethical Committee. Gradient was approved to select sperm with longer telomere length. Results: STL is positively associated with progressive sperm motility. Conclusion: Thus, the use of assisted reproductive technol-ogies to choose sperm with longer telomeres boosts the success rate of pregnancies and births, which in turn enhances the prog-eny's longevity.