Microalgae culture media should be cost-effective, enable high growth, meet precise requirements, and be readily available. In this study, the effect of different levels of sodium bicarbonate (SB) and Soaked grated beans (SGB) in the growth medium on the biochemical components (protein, carbohydrate, lipid, fatty acids, and amino acids) of Nannochloropsis oceanica was evaluated compared to standard Gaillard F/2 medium. Treatments were performed as follows: - control (OCO): Gaillard F/2 medium, OD1: 75 % of SB+25% of SGB, OD2: 50% SB+50% of SGB and OD3: 25%SB+75%of SGB. The results obtained revealed that the biochemical components of N. oceanica are affected by the level of sodium bicarbonate and bean sprout infusion. The highest protein and carbohydrate contents were obtained from the OD3 treatment, the highest lipid content from the OD1 treatment and the highest EAA content (54.41%) were obtained using OD3 medium compared to the control group (100% F/2). The highest biomass productivity was obtained from OD3 treatment followed by OD2 and the highest total saturated fatty acids (TSFA) content of N. oceanica was recorded by OD2 medium.