During this study, the physical and chemical parameters of the Tigris River water were measured at four sites along the Tigris River extending between Mosul and Badoush Dams for the period from September 2023 to February 2024 to evaluate the Tigris River water and its suitability for human use at the sites (1, 2, 3 & 4). The physical, chemical and biological parameters were studied, such as pH, air and water temperature, electrical conductivity (EC), total dissolved solids (TDS), total alkalinity, active phosphate (PO₄), nitrite (NO₂), chloride (Cl^ــ), active silica (SiO₂), and total plate count (TPC). Some of them were conducted in the field and others in the laboratory. It was found that most of the parameters that were studied are consistent with the international and Iraqi specifications for drinking water, except for the total platelet count (TPC), especially at station No. (4), which was noticeably high, reaching 12.9*10² cells/ ml. The pH values ​​ranged from 6.5-7.37, water temperature from 7-24 degrees Celsius, while the electrical conductivity (EC) was at a rate of 251µS/ cm. The average value of total dissolved solids (TDS) was 166.35mg/ L, and the percentage of dissolved oxygen (DO) was at a rate of 8.94mg/ L. Silica levels ranged from 1.00-2.23μg/ L, while the rate of effective phosphate was 0.04μg/ L, and the nitrite values ​​were at a rate of 0.254μg/ L.