Fish resources are critical for the livelihoods of local communities in Ambon Bay, which has historically served as a key area for small-scale bait fisheries, particularly small pelagic species. However, these resources have experienced a significant decline, with species now becoming increasingly scarce. This reduction is primarily attributed to anthropogenic impacts and overexploitation. Addressing this decline requires a scientific understanding of fish resource utilization and the development of community awareness regarding sustainable practices, though research in this area remains limited. This study employed a combination of ichthyofauna diversity surveys, participant observations, and forty-two interviews conducted in four locations across Ambon Bay. Respondents included fishermen, government officials, researchers, and academics. The involvement of these stakeholders provided insights into local knowledge, traditional practices, and the community's role in fish resource management. Observations focused on fish reproductive maturity, habitat conditions, and fishing seasons, creating a comprehensive overview of resource utilization. The findings indicated a notable decline in the abundance, species variety, and size of fish resources in the bay. Community-based initiatives to shift from capture fisheries to aquaculture, proposals for the transplantation of Stolephorus indicus and Encrasicholina heteroloba, and the enforcement of regional regulations emerge as viable strategies for mitigation. This study underscored the importance of collaborative efforts among stakeholders to ensure the sustainable utilization of fish resources in Ambon Bay, aiming to secure ecological and socioeconomic benefits for future generations.