The production of the biosurfactants (BS) from industrial organic waste has gaining significant interest due to its potential to reduce production costs. The production and properties of biosurfactants have been investigated from eight bacterial strains of Bacillus sp. and Peribacillus frigoritolerans. Which were previously isolated from biofouling anion exchange resin used in electric power plants (Egypt). Waste industrial oil (transformer and lubricating) was used as alternative cheap carbon sources for the biosurfactants production. Screening and selection of biosurfactant producer(s), were examined by using different parameters includes; blood hemolysis test, surface tension measurements, oil spreading test, emulsification index and biosurfactant yield. The stability values showed significant emulsification results at pH 12 resulting of Bacillus licheniformus BaDB24 (75.6%) > Bacillus cereus SH16 (63.05%)> Peribacillus Frigoritolerans (29%). The stability of temperature at 75°C that showed emulsification values resulting for Peribacillus Frigoritolerans (60.5%)> Bacillus licheniformus BaDB24 (35.2%)> Bacillus cereus SH16 (25%). The stability of salinity at 14% resulting that showed emulsification values of Peribacillus Frigoritolerans (60.8%)> Bacillus licheniformus BaDB24 (58.95%)> Bacillus cereus SH 16 (41.7%). The microorganisms studied were able to produce a biosurfactant from industrial waste oil. From tested strains, two bacteria of Bacillus licheniformus BaDB24 and Peribacillus frigoritolerans were chosen to be the potential choice for biosurfactant production.