Enhancing health and production efficiency of poultry industry using natural products is now being applied as an alternative for synthetic compounds and antibiotics. Lactobacillus recognized for their positive impact on gut health and immunity due to their direct interaction with the gut microbiota, which is essential for a robust immune system. On the other hand, methionine is an essential amino acid in poultry nutrition, playing a crucial role in protein synthesis, growth, and overall health. The present study was designed to present and evaluate the effects of DL-methionine. and lactobacillus as dietary supplements on the growth performance, nutrients digestibility and blood constituents of poultry. The study was conducted on 50 one-day old Hubbard chicks. The chicks were assigned to five groups. 1^st group (control negative) was fed on a basal diet for 4 successive weeks. 2^nd group was fed on a basal diet mixed with DL-methionine at a dose of (1 g/ kg diet). 3^rd group was fed on a basal diet mixed with lactobacillus at a dose of (1 ml / liter of water). 4^th group was fed on a basal diet mixed with DL-methionine at a dose of (2 g/ kg diet). 5^rd group was fed on a basal diet mixed with lactobacillus at a dose of (2 ml / liter of water). The results showed that DL-methionine (specifically the higher dose 2 g/ Kg feed) induced a significant decrease in feed conversion, while total proteins and albumin levels were increased in both groups that consumed 1 and 2 ml lactobacillus /liter of water. Gamma globulin was increased significantly in the group that was supplied with 1 ml lactobacillus/liter of water. Superoxide dismutase showed significant increase in groups given DL-methionine with dose 2 g/kg diet and lactobacillus with dose 2ml /liter of water compared to control group. Glutathione showed significant elevation in the group given DL-methionine with dose 2 g/kg diet.