Background: Urinary tract infections are the most frequently occurring infections associated with healthcare in the Intensive Care Unit (ICU). Prolonged catheterization stands out as the primary risk factor for developing UTIs. Aim: compare chlorohexidine versus povidine iodine in the prevention of catheter associated urinary tract infection among critically ill patients. Design: Quasi experimental study was carried out at Damanhur Medical National institute, Egypt. Sample: a Purposive sample of 180 patients selected and divided equally to two groups, Group (A) used chlorhexidine gluconate, Group (B) used 10 % povidone Iodine solution in the insertion of urinary catheter. Results: Majority of the sample were male and 40% of povidine iodine group and 43.3% of chlorohexidine group were in age group from (31 < 41). Also, There was a highly statistically significant difference between the povidine iodine group and chlorohexiedene group regarding total catheter associated urinary tract infection assessment profile as chlorohexidene group is less symptoms than povidine iodine group with p-value = (0.000). Conclusion: using chlorhexidine gluconate in insertion of urinary catheter was statistically significant difference in reducing catheter associated urinary tract infection among critical patients than povidine iodine. Recommendation: Comparison between soap and Chlorohexidine in reducing Catheter associated urinary tract infection.