Appetite regulation involves a complex interaction of physiological, psychological, and hormonal factors. Thyroid-stimulating hormone (TSH), primarily responsible for regulating thyroid function, has recently been associated with appetite control. This study explores the relationship between serum TSH levels and appetite in healthy adults. A cross-sectional study was conducted on 350 adults aged 18-60, recruited from Azadi Teaching Hospital, Duhok, Iraq. Participants with thyroid disorders or metabolic diseases were excluded. Data collection involved a comprehensive questionnaire, face-to-face interviews, and review of medical records. Statistical analysis, including Pearson's correlation, were used to assess associations between serum TSH levels and appetite-related parameters. The study cohort was predominantly female (82.5%) and unemployed (83.4%). TSH levels exhibited a positive correlation with appetite changes (r = 0.1404, p = 0.0085). Creatinine levels showed a moderate positive correlation with age (r = 0.1796, p = 0.0007), while appetite changes did not significantly correlate with age (r = -0.05179, p = 0.9230). This study suggests a potential link between TSH levels and appetite regulation, contributing to a broader understanding of metabolic health. Further research is needed to explore the underlying mechanisms and clinical implications of these findings.