Abstract: Background: Lower limb amputation (LLA) is usually performed as a lifesaving procedure; it has a significant and dramatic change in a person's life which has an effect on quality of life of patients due to the physical activity limitations immediately after amputation and long-term implications in different aspects of life. The purpose for the study was to determine the effectiveness of nursing intervention on phantom pain, activities of daily living and quality of life among patients with amputation. Design: A quasi-experimental research design was utilized. Setting: the study was conducted at the general surgical department and rehabilitation unit in Menoufia University Hospital, Menoufia Governorate, Egypt. Sample: A consecutive sample of 60 adult patients were selected. Four instruments were used in data collection; 1) A Structured Interviewing Questionnaire, 2) Modified Barthel Index (MBI),3) World Health Organization of Quality-of-Life Brief (WHOQOL-Brief), and visual analogue pain scale. Results: 30% of both groups were male with mean grades of pain 4.73±2.77 and 5.90±3.35 of study and control groups respectively at post-intervention. Conclusion: patients with amputation have obvious improvement in pain score, activities of daily living and quality-of-life. Recommendation: The Continuous educational and training programs should be provided for patients with lower limb amputation on regular basis to improve their physical and psychological health and quality of life.