Background: University laboratories involve various hazards during the research and course activities, which might affect health and safety of students, including chemical, physical, electrical, and biological risk factors. accounting for both death and disability, the fraction of the global burden of disease due to hazards is 24% (WHO 2019). Aim of the study: This study aims to assess awareness of university students regarding laboratory hazards at Fayoum University. Design: A descriptive analytical design was used to conduct this study. Setting: The study was carried out at 4 Facilities are (Agriculture ,science ,pharmacy and Engineering )affiliated of El-Fayoum University. Sample: A Stratified random sample was used and including strata students from 2nd, 3rd and 4th grade of Faculties. Tools of data collection: it consisted of two tools used in data collection: 1 st tool consists of 3 parts demographic characteristics of university students, University Students knowledge regarding laboratory hazards, and University students precaution or safety measures regarding laboratory hazards.2nd tool: observational checklist sheet for laboratory include two parts it was standardized tool comes from (NIHDRM, 2016) consists of 2 parts Self-Reported using personal protective measures and Health standers for laboratory. Result :74% of studied students were had satisfactory knowledge about laboratory hazards. While 24% of studied students were had unsatisfactory knowledge about laboratory hazards. 87% of them had unsafe self-reported protective measures about laboratory hazards. While 95.3% of them had unsafe total standers for laboratory Health and self-reported protective measures about laboratory hazards. Additionally there was statistically significant reference between studied students practice and personal protective /safety measures. Also there was statistically significant reference between studied student's and knowledge & precaution or safety measures toward laboratory hazards. Conclusion: In the light of the current study findings, it can be concluded that, additionally there was statistically significant reference between studied students practice and personal protective /safety measures. Also there was statistically significant reference between studied student's and knowledge & precaution or safety measures toward laboratory hazards. Recommendations: Based on the current study finding the following recommendations were proposed: Developing and implementing of health education programs to increase their knowledge, practice of students regarding laboratory hazards .