Background: Mothers are primarily responsible for protecting and maintaining the health of their family members. They can be a role model for their family member and affect positively their health behaviors for protection against the infection with COVID-19. The study aimed to: assess mothers perception regarding preventive measures of COVID-19. Research design: descriptive design was utilized to achieve the aim of this study. Setting: The study was conducted at Elamiria Medical  Center in Elzaton Zone affiliated to Ministry of Health . Sample: A Purposive sample of 264 enrolled at the above mentioned setting was involved in this study. Tools: structural interviewing questionnaire: It was included three parts as following: Part (1): Socio demographic data of mothers. Part (2): Mothers Knowledge related to preventive measures of COVID-19. Part 3: Mothers reported practices regarding prevention of COVID-19 virus The second tool: Mothers attitude regarding preventive measures of COVID-19 virus infection assessed through Likert Rating scale. Results: Findings of the present study showed that 63.6% of the studied mothers, had satisfactory level of total knowledge regarding COVID-19 ,68.9% of the studied mothers had healthy reported practices regarding their total reported practices about preventive measure for COVID-19 while31.1% of their total reported practices were unhealthy,71.6% of the studied mothers had positive attitude regarding preventive measure for COVID-19  . Conclusion there was a highly statistical significant difference between studied mother's total attitude and their reported practices regarding preventive measure of COVID-19 .Also, there was highly statistical significant difference between studied mother's total knowledge and total reported practices regarding preventive measure of COVID-19. Recommendation: Design educational program by family and community health nursing to increase mothers perception in MCH center in order to upgrading mothers knowledge, practices and attitude regarding preventive measures of COVID-19.  disseminate multiple communication approaches, digital, paper, prochures , phone messages, etc .