Background: severely ill patients have greater risk of acquiring nosocomial infection in intensive care unit. Nurse's compliance with infection control and sterile technique principles can prevent such problem in critical care unit. Aim: This study aimed to assess the extent of the nursing team commitment to infection control protocol in critical cases units. Design: A descriptive exploratory research. Setting: Critical care units at main Assiut university hospital. Subjects: A convenience sample of (50) nurses within 3 months.  Tools: two tools were used: Tool (I): - Nurses' Interviewing questionnaire. Tool (II): Nurses Observation checklist. Results: 62.0% of the studied nurses had unsatisfactory level of knowledge and 86.0% of them had unsatisfactory level of practice regarding commitment to infection control protocol in critical care unit.  Conclusion: More than two third of studied nurses had unsatisfactory level of knowledge and majority of them had unsatisfactory level of practice regarding commitment of infection control protocol in critical care units.  In addition, there was a significant negative correlation between nurses' Knowledge and practice regarding commitment to infection control protocol in critical care units.  Recommendation Further research is recommended to evaluate the effect of training program of nurse's performance regarding standard precautions for infection control