This study, conducted at Sakha Agricultural Research Station, Kafr El-Sheikh, Egypt, during the 2020/2021 and 2021/2022 growing seasons, investigated the effects of salinity stress on grain yield, its components, physiological and grain quality traits in 21 bread wheat genotypes (Triticum aestivum L.), including18 lines and 3 check cultivars to assess the genetic variability and identify salt-tolerant wheat genotypes. To achieve these objectives, the genotypes were evaluated under normal and saline soil conditions. Salinity stress significantly reduced most traits across all genotypes except for some physiological traits (MDA, Proline, and Na/K) and wet dry gluten percentage which exhibited increases under stress. Significant variation was observed among the genotypes for their performance under saline conditions. Based on discriminant scores Lines 5, 8, 12, 13, 18, Misr 4, and Sakha 95 are suitable and demonstrate normal physiological responses to avoid salinity effects. Line 4, Line 2, Line 9, Line 15, Line 3, and Line 7 and Line 6 showed moderate tolerance to salinity. In contrast, Lines 1, 10, 11, 14, 16, 17, and Sakha 96 are classified as sensitive genotypes to salinity stress.