Biological control using lactic acid bacteria (LAB) is an environmentally friendly strategy for phytopathpgenic fungi management. This study contains bacterial isolates which were isolated from yoghurt and tested to antagonize Fusarium spp. usually attacks the germinated seeds and seedlings causing pre-and post-emergence damping-off. Therefore, the superior phytopathogenic fungi invade several plants as Fusarium oxysporum and Fusarium solani were chosen to evaluate LAB as a biocontrol agent for them. Of the five lactic acid bacterial isolates (LAB-1, LAB-2, LAB-3, LAB-4, and LAB-5), one isolate (LAB-3) recorded high inhibition linear growth for fusarium-pathogens (80.5% and 87.6% respectively). LAB-3 was chosen for the study of main characters, the results showed that the isolate was Gram-positive, non-sporulating, rod-shaped (bacilli), catalase-negative, microaerophilic, fermentated glucose, lactose, and other sugars. Therefore, this isolate (LAB-3) was identified as members of the Genus Lactobacillus. In vitro, these bacteria are very important because producing chitinases that are the main fungal cell wall structure. This enzyme produced by lactic acid bacteria can be safely and easily used in plant protection for the inhibition of fungal pathogens. In addition, the activity of antifungal metabolites is produced by LAB. In vivo, at the time of planting when treating plant seed with LAB-3 and soil with Fusarium showed increased seedling survival from 25 to 85% and from 40 to 80% respectively, in natural soil. This work indicated that this bacterium (LAB) is environmentally safe and can be used successfully to control phytopathogenic fungi.