Background: Keratoplasty or corneal transplantation is a common procedure for restoring vision in patients with corneal diseases. Despite its success, effective post-operative care is essential to prevent complications. Inadequate patient knowledge and improper self-care can negatively impact outcomes. Aim: This study aimed to evaluate the impact of educational program on post-operative health outcomes in patients undergoing keratoplasty at the Ophthalmology Center, Mansoura University, Egypt. Methods: A quasi-experimental design was used involving 40 adult patients who undergoing corneal transplantation. Tools: Two tools were used for data collection; the first tool was two sections involved; Patient Demographic parameters, and Keratoplasty Knowledge Questionnaire, the second tool is Patient's Health Outcomes Questionnaire: This tool, which has two sections; Post-Operative Keratoplasty complications or Problems, and Self-Care Practices Questionnaire. Validity and reliability of tools were ensured through pilot testing and expert review. Results: Post-intervention results indicated significant improvements in patient knowledge (p < 0.001). Adherence to self-care practices better attained (p < 0.001). Post-operative complications decreased significantly including symptoms of foreign body sensation were reported (p < 0.05), photophobia decreased and reported pain significantly (p < 0.05). Conclusion: Significant improvements in postoperative outcomes were attained by keratoplasty patients by increasing knowledge and improving practices when an educational program was implemented. Recommendations: Future research must attempt to include longer follow-up periods, more larger and diverse samples for determining the long-term efficacy and stability of the program's benefits.