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410659

Identification of Hypervariable Domains in 12SrDNA for Enhanced Fish Species Barcoding from the Red Sea Regions of Egypt and Yemen

Article

Last updated: 08 Feb 2025

OverviewSimilar Items

Subjects

-

Tags

Zoology

Abstract

Ribosomal RNA (rRNA) genes, particularly 12SrDNA, have proven to be effective barcodes for fish species identification due to their secondary structure domains that exhibit interspecific variability. This study focused on identifying hypervariable domains within the 12SrDNA gene that contribute to its barcoding efficiency. Fish samples from Egypt and Yemen, representing various orders and families, were analyzed using 12SrDNA and COI gene sequencing. Four hypervariable domains (D2, D3, D5, D7) within 12SrDNA showed the highest divergence among species. Concatenating these domains significantly increased interspecific genetic distances. COI and 12SrDNA barcodes revealed 3 misidentified species (Plectorhinchus sordidus, Mulloidichthys flavolineatus, and Lethrinus nebulosus.), emphasizing the importance of focusing on specific hypervariable domains for accurate species identification.

DOI

10.21608/sjsci.2024.312092.1219

Keywords

COI gene, DNA barcoding, Hypervariable domains, Fish species, 12SrDNA, Red Sea

Authors

First Name

Asmaa

Last Name

Galal-Khallaf

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.

Email

asmaakhallaf@science.menofia.edu.eg

City

-

Orcid

-

First Name

Aya

Last Name

Elberri

MiddleName

Ibrahim

Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.

Email

aya_elberry90@yahoo.com

City

-

Orcid

-

First Name

Omir

Last Name

Rabie

MiddleName

AbdelWahab

Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.

Email

omirabdelwahhab@yahoo.com

City

-

Orcid

-

First Name

Taha

Last Name

Baker

MiddleName

S. S.

Affiliation

Department of Biology, College of Education, Ataq Shabowah University, Yemen.

Email

taha_baker2002@yahoo.com

City

-

Orcid

-

First Name

Abdelaziz El-Sayed

Last Name

El-Sayed

MiddleName

A. A.

Affiliation

Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt., Department of Zoology, Faculty of Science, Islamic University of Madinah, Saudi Arabia.

Email

abosharaf63@yahoo.com

City

-

Orcid

-

First Name

Hamdy

Last Name

Soliman

MiddleName

A. M.

Affiliation

Department of Zoology, Faculty of Science, Sohag University, Egypt.

Email

hamdy90@yahoo.com

City

-

Orcid

-

First Name

Khaled

Last Name

Mohammed-Geba

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.

Email

kgeba@umces.edu

City

-

Orcid

-

Volume

10

Article Issue

1

Related Issue

52890

Issue Date

2025-03-01

Receive Date

2024-08-13

Publish Date

2025-03-01

Page Start

88

Page End

94

Print ISSN

2357-0938

Online ISSN

2974-4296

Article File

SJSCI_Volume 10_Issue 1_Pages 88-94.pdf

PDF . 2.0MB

Link

https://sjsci.journals.ekb.eg/article_410659.html

Detail API

http://journals.ekb.eg?_action=service&article_code=410659

Order

410,659

Type

Regular Articles

Type Code

2,359

Publication Type

Journal

Publication Title

Sohag Journal of Sciences

Publication Link

https://sjsci.journals.ekb.eg/

MainTitle

Identification of Hypervariable Domains in 12SrDNA for Enhanced Fish Species Barcoding from the Red Sea Regions of Egypt and Yemen

Details

Type

Article

Created At

08 Feb 2025