The spatial distribution of ichthyofauna in seagrass habitats is influenced by habitat characteristics, its proximity to other habitats, and the daily temporal distribution of fish communities, which varies with orientation. This study aimed to analyze the crepuscular distribution of ichthyofauna by comparing the presence of species, families, and orders, as well as the structure of fish communities, between the dawn and dusk periods on Gili Noko, Bawean Island, East Java, Indonesia. The study was conducted in June 2024, with sampling during the dawn period at sunrise (5:00–6:00 AM) and the dusk period at sunset (5:00–6:00 PM), using bottom gillnets to collect fish samples. The data collected were analyzed for species presence, species composition, family and order distribution, and community structure (i.e., diversity index, evenness, and dominance index). The results were presented in tabular and graphical formats. To assess the similarities in species, families, and orders between the two crepuscular periods, a Bray-Curtis analysis was performed. The study recorded 76 individuals, 34 species, 15 families, and 10 orders. The highest number of individuals, species, families, orders, and community structures were found during the dusk period compared to the dawn period. The similarity of species between the two crepuscular periods was moderate, indicating differences in species presence between the two times. The diversity of life phases and feeding habits of the fish highlights the role of seagrass beds as both a nursery and a feeding ground. Consequently, conservation efforts aiming at protecting seagrass ecosystems are crucial for preserving fish diversity and supporting sustainable fisheries and ecotourism.