Background: Children's safety presents a significant challenge, as hospitalized children face a three - times higher likelihood of harm compared to adults. Aim: To assess safety measures provided by nurses for mechanically ventilated children. Design: A descriptive research design was applied. Subjects: All nurses (50) who were working in pediatric intensive care unit. Setting: This study was carried out at the pediatric intensive care unit in Assiut University Children's Hospital. Tools: Two tools were employed to gather the needed data: interview questionnaire sheet, and observational checklists. Results: More than two- fifths (42%) of the studied nurses had fair knowledge about safety measures for mechanically ventilated children, while 30% and 28% of them had poor and good knowledge, with two-thirds (66%) of them showed unsatisfactory practical skills in this area. A significant positive correlation (r = 0.297*) was observed between their overall knowledge and practical performance and a statistically significant difference was detected (Pv =0.036*). Conclusion: There was a lack of nurses' knowledge and practices regarding safety measures provided for mechanically ventilated children. So, the researcher Recommended that educational programs and advanced training courses should be developed and implemented based on evidence-based guidelines and the specific needs of staff nurses to enhance their knowledge and practices regarding safety measures for mechanically ventilated children.