The aim of this study is to examine the effectiveness of pulsed low-dose-rate radiation therapy (PLDR) in controlling hepatic cancer. Hepatocarcinogenesis was induced in rats using diethylnitrosamine (DENA). The rats were classified into 4 groups, negative control group, positive control group, conventional radiation group (CRT) and PLDR group. Both irradiated groups received 6Gy to the liver using 6MV beams. Body weight was observed daily. Tumor volume was measured using computed tomography scan (CT). Liver tissues were subjected to histopathology. Alfa fetoprotein (AFP) and interleukin 6 (IL-6) level were assessed. Both CRT and PLDR techniques could significantly inhibit hepatic tumour growth when compared with non–irradiated group (P value < 0.05), PLDR technique showed better results compared to the CRT technique, but the difference was not significant, P >0.05. PLDR group showed a continuous increase in weight, while CRT group showed gradually weight loss until the 5th day. Both groups demonstrated a significant decrease in AFP and IL-6 serum level compared to the positive group. The estimated AFP and IL-6 in PLDR were lower than that of CRT (P=0.16 and P=0.001, respectively). The average levels of serum AFP were 0.554 ±0.11, 2.52± 0.23 ,1.258± 0.39 and 0.976± 0.35 and for IL-6 were 16.01± 2.5 ,46.9±2.9, 27.5±1.5 and 22.5±1.03 for negative control, positive control, CRT and PLDR, respectively. Histological examination indicated more liver cell degeneration with CRT. Hepatic tumors could be controlled with PLDR at least as successfully as CRT, with a beneficial advantage of showing significantly less normal tissue toxicity.