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409313

The Immunomodulatory Effect of Anti-Cancer Drug Cisplatin on Colon Cancer SW480 Cell Line

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Cisplatin is a potent chemotherapeutic drug that is widely used and effective in cancer treatment, but its effect is rarely studied in the context of the dynamics of cancer cells. Objectives: This study aims to clarify the effect of cisplatin on a cancer cell line under normal circumstances, using untreated cells as a control. The effect of cisplatin on controlling some key biomarkers is investigated including CD147, IL-17 and ACE-2. Methodology: The cancer cell line was treated separately with different concentrations of cisplatin (6.25, 12.5, 25, 50 and 100 µg/ml) as compared with the same cell line. The untreated cells were used as control cells. The concentrations of CD147, IL-17 and ACE-2 were estimated by ELISA kits. Results: In the presence of cisplatin, there is a clear downregulation in the expression of ACE-2 and CD147 in a dose-dependent manner, and the effect is significantly different (p<0.05) at concentrations above 25 µg/ml. The downregulation of ACE-2 and CD147 was significantly enhanced, and the difference was significant (p<0.05) at much lower concentrations of 16.5 µg/ml. The expression of IL-17 was inversely proportional to the concentration of cisplatin. At lower concentrations of cisplatin, IL-17 was suppressed, but this effect was limited to higher doses, indicating a threshold effect for IL-17 suppression. Conclusion: Cisplatin downregulated ACE-2 and CD147 in a cancer cell line. These findings suggest that cisplatin may be beneficial in treating patients with cancer, and the mechanism of action may include modulation of key cellular receptors and inflammatory cytokines involved in tumor pathophysiology.

DOI

10.21608/ejmm.2025.350644.1436

Keywords

ACE-2, Cancer, CD147, Chemotherapy, Cisplatin

Authors

First Name

Alaa

Last Name

Hameed

MiddleName

K.

Affiliation

Department of Laboratories, Al-Hussein Teaching Hospital, Al-Muthanna Health Department Employee, Al-Muthanna City, Iraq

Email

alaaadhemameed@yahoo.com

City

najaf

Orcid

-

First Name

Ifad

Last Name

Abd Al-Shibly

MiddleName

K.

Affiliation

Department of Microbiology, College of Medicine, Babylon University, Hilla, Iraq

Email

ifaderimbdal-shibly@yahho.com

City

najaf

Orcid

-

First Name

Rana

Last Name

Ghaleb

MiddleName

A.

Affiliation

Department of Human Anatomy, College of Medicine, Babylon University, Hilla, Iraq

Email

ranahaleb222@yahoo.com

City

najaf

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-01-10

Publish Date

2025-04-01

Page Start

319

Page End

324

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 319-324.pdf

PDF . 506.4kB

Link

https://ejmm.journals.ekb.eg/article_409313.html

Detail API

http://journals.ekb.eg?_action=service&article_code=409313

Order

38

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The Immunomodulatory Effect of Anti-Cancer Drug Cisplatin on Colon Cancer SW480 Cell Line

Details

Type

Article

Created At

08 Feb 2025