The aim of this experiment is to study the effect of different concentrations of BAP plant growth regulator on the chemical composition of Lepidium sativum plantlets in the case of micropropagation in vitro and chemical analysis of them, and to compare these results with the results of the chemical analysis in the case of cultivation of L. sativum in the field. In vitro, MS media was used supplemented with different concentrations of cytokinin BAP at (0.0, 1, 2, 3, 4, 5 mg/L BAP) to produce the plantlets from the seeds. By comparing the chemical analysis of the chemical components of the plantlets produced in vitro and the chemical analysis of the plants those were grown in the field, the comparison revealed a higher value of chemical compounds in the field plants compared to the chemical compositions resulting from micropropagation of the plantlets in vitro. From our study we obtained the following, the maximum values of shoots number, plantlet length, plantlet weight, plantlet wide, was recorded with 3.0 mg/L BAP and the maximum value of reading spad, chlorophyll a, b and chlorophyll a+b were recorded with 5.0 mg/L BAP, on the other hand the maximum values of carotenoids, total phenolics, flavonoids contents and antioxidants activity in plantlets were recorded with 4 mg/L BAP. While cultivation of Lepidium sativum in the field gave values in chemical compounds greater than those obtained when cultivation in the laboratory.