Background: Breast tissue malignant cells are the source of breast cancer, which has emerged as one of the major medical issues with a socioeconomic impact, particularly for women. Oxidative stress is one of the many variables that contribute to the beginning, development, and invasion of breast cancer Objective: This investigation was conducted to assess the utility of cytokine and serum protein oxidation indicators in the detection of breast cancer.. Methodology: 88 females with pathologically and clinically diagnosed breast cancer (mean age = 58 ± 6.2 years) were selected through self-selection. There were 58 female controls in the study. Protein oxidation indicators and cytokines were evaluated immunoenzymatically in the serum from the patients and controls. Advanced oxidation protein products (3-NT), cytokines (IL-1, IL-8, IL-10, IL-12, and IL-17), and serum were all detected using an ELISA assay. Results: The levels of 3-nitrotyrosine (3-NT) and advanced oxidation protein products (AOPP) were significantly higher in the study group than in the control group (P<0.01) compared to the control group, serum levels of all cytokines (IL-1, IL-8, IL-10, IL-12 and IL-17)were significantly higher in the study group than in the control group (P<0.001,) 0.0033, 0.004, 0.001, < 0.001)respectively. Conclusions: High protein oxidation is an important risk factor for breast cancer. The findings suggest that the pathogenesis of breast cancer was related to an aberrant imbalance of cytokine production, and cytokine profiles may be used as early diagnostic markers