Mastitis in dairy herds is of considerable significance, among various etiological agents, Staphylococcus aureus is the most predominant cause of subclinical mastitis in dairy animals. Increased trends in antimicrobial-resistant bacteria and failure of antibiotics lead to increased incentives to look for alternative control measures such as herbal extracts and their active compounds to be applied in manage and control of causative bacterial diseases. This study was designed to determine the antimicrobial efficacy of pomegranate peel extracts against S. aureus isolated from mastitis. Analysis of its content of active ingredients was taken into account using GC-Mass. The disk diffusion assay was used to estimate the antibacterial effects in the S. aureus strains. The GC-MS results showed that pomegranate peel extract contained 40 components, 14 of which have documented antibacterial activity including phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. Five of which exhibited resistance to beta-lactams. Pomegranate peel extract was able to inhibit the growth of S. aureus at a concentration of 3.125 mg/ml. Scanning electron microscopy indicated the pomegranate peel extracts disrupted the bacterial cell wall causing cell deformations that would decrease their pathogenicity and could control the disease efficiently. Pomegranate peel extracts represent a promising alternative treatment for mastitis in dairy animals.