Poor storage and Aflatoxin contamination of nuts is an increasing concern to the consumer's health so the current study was undertaken to analyses the effect of poor storage on chemical composition, aflatoxin and experimental rats in nuts (peanut and almond). Peanut and almond were store 18 month and The effect of poor storage nuts was investigated on experimental rats Thirty white albino rats were randomly classified into five groups: (1) control negative, (2&3) group will be fed on100g fresh and storage peanut /kg diet, respectively. (4&5) group will be fed on 100g fresh and storage almonds /kg diet, respectively. The results showed the find fungi in peanuts and almonds after poor storage, which led to the appearance aflatoxin B1 and B2 were higher in peanut about almond after 18months storage. The results suggested that protein and fat decreased significantly, while highly significant increase was observed in moisture content.. Showed in final, body weight reduce and food intake as compared to control negative group. Almond and peanut the higher level serum ALT, AST and ALP in the store group unlike fresh group. showed significant increase in serum Uric acid, Urea nitrogen and creatinine compared to control negative group