The objectives of this study were to comparison between tow different available household wastes and investigate affect the volume of biogas production. Determine the most appropriate mixing ratio between waste and water and also determine the appropriate filling ratio for the fermenter. Microbiological evaluation of fermented wastes mixture were done. The experiment includes three variables. The first variable was the type of waste which included two base crops, which were rotten tomatoes fruits (To) and Jute-mallow green part (Ma). The second variable was the filling ratio (F) with two percentages 50% ( F1 ) and 75% ( F2 ) of full tank size. The third variable was the volumetric mixing ratio (M) between water: waste and includes ( 1:1 as M 1 ), (1:2 as M 2 ), ( 2:1 as M 3 ) . The experimental results showed that the best treatment produced the most amount of biogas , whether in Jute-mallow ( Corchorus olitorus ) green part waste or rotten tomato fruit, were (M2 F2), which was (the volumetric mixing ratio between water: waste (1:2) + 75% filling ratio), so that it was 25454 cm3 per Jute-mallow green part and 32905 cm3 in rotten tomato fruit. The results also show that the biogas produced from Jute-mallow green part contains a large percentage of methane gas when compared to the biogas produced from rotten tomato fruit, so that the highest percentage of methane gas produced from the samples used for Jute-mallow green part reached 32.5% at the 50% filling ratio and 43.39% in 75% filling ratio, while the highest percentage was in the samples used for rotten tomato fruit 0.26% in the 50% filling ratio and 0.16% in 75% filling ratio. The highest value of total bacterial count was in treatment (To,M2,F1) being 103.05 (x107cfu/ml). The highest fungal count was in treatment (To,M1,F2) being 26.25 (x107cfu/ml). Amylolitic and Protiolytic count were in treatment (Ma,M3,F2) being 42 (x107cfu/ml) and 41.25 (x107cfu/ml), respectively. The highest value of Lipolytic microbes count was in treatment (To,M3,F2) being 47(x107cfu/ml). The highest value of Cellulolytic microbes count was in treatment (Ma,M2,F1) >1100 (x107cfu/ml).