Cr(III), Zn(II), Ni(II) and Cu(II) complexes of a symmetric Schiff base ligand (L), (Z)-N'-((1E,2E)-1-(2-(p-tolyl)hydrazineylidene)propan-2-ylidene)-2-((E)-1-(2-(p-tolyl)hydrazineylidene)propan-2-ylidene)hydrazine-1-carbohydrazide has been synthesized. Elemental analysis, UV-Visible, FT-IR, mass spectra and conductometric techniques were used to characterise the ligand and its metal complexes. The microorganisms utilized in this study involve Streptococcus mutans, Staphylococcus Aureus (as Gram-positive bacteria), Escherichia Col and Klebsiella pneumonia (as Gram-negative bacteria) and Aspergillus Nigar and Candida albicans (as fungi). Comparing all of the studied species, Cu(II) complex exhibits the highest activity versus tested fungi.