Introduction: The use of technology in education and online learning is progressively increasing and this has its merits and demerits for medical students. One of the demerits is developing technostress which is characterized by anxiety and frustration. This certainly will affect students' wellbeing and performance. Aim of Work: To estimate the levels of different technostress creators among Mansoura University medical students and their associated factors. Materials and Methods: This is a cross-sectional study that was carried on a sample of 814 students from faculty of Medicine, Mansoura University, Egypt. A self-administered questionnaire was constructed using google form to inquire about sociodemographic data and technostress creators. The quality of Wi-Fi connection, daily hours of technology use, and duration of use by years, were also inquired about and considered in relation to different technostress creators. Results: Among the studied technostress domains, techno-uncertainty (3.35 ± 0.78), techno-invasion (3.29 ± 0.766) and techno-overload (3.15 ± 0.59), were the highest technostress creators among the students. More techno-overload was found in students with more daily hours of technology use. More techno-invasion was detected among students with longer years of Information and Communication Technologies (ICT) experience. Techno-insecurity was more among students with poor Wi-Fi connections. Students using technology for less than 5 hours daily had more techno-insecurity. Conclusion and Recommendations: Education using the current technological methods is associated with stress among medical students. Further studies can be performed to investigate the effects of students training on the use of ICT on decreasing the associated technostress and improving students' performance.