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384787

A Recent Approach for In-vitro Evaluation of the Inactivated Rift Valley Fever Vaccine

Article

Last updated: 25 Feb 2025

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Subjects

-

Tags

Veterinary Parasitology & Microbiology

Abstract

With the global movement toward more widespread implementation of the 3Rs principle (Replacement, Reduction, Refinement of animals use) in bioscience, substantial efforts have been made to advance alternative in-vitro potency assays thus eliminating the disadvantages of the animal-based models regarding the number of used animals, assay time, costs and variability of results. The objective of the present study is to assess the use of sandwich ELISA as an alternative in-vitro potency assay for quality control and release testing of the inactivated RVF vaccine. Three inactivated Rift Valley fever vaccine batch samples in addition to a reference sample were evaluated by both in vitro and in-vivo methods. The results demonstrated the correlation between the antigenicity measured by in-vitro potency assay and the immunogenicity assessed by the current in-vivo assays. In conclusion, the in-vitro potency assay proved to be predictive to the in-vivo immunogenicity, and can be a reliable alternative not only provide a mean for ethical research by replacing animals use but strongly impacting the assay time, cost and efforts and pave the way for adoption of the consistency approach as a strategy for vaccine quality.

DOI

10.21608/ejvs.2024.287303.2063

Keywords

Rift Valley fever, 3Rs, In-Vivo, In-vitro

Authors

First Name

Noha

Last Name

Fayed

MiddleName

Ezz-Eldeen

Affiliation

Rift valley fever,veterinary serum and vaccine research institute ,Abbassia,Cairo

Email

noha.ezzeldeen86@gmail.com

City

Cairo

Orcid

-

First Name

Diana M.

Last Name

Abulmagd

MiddleName

-

Affiliation

Rift Valley Fever Vaccine Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt

Email

diana2010mohamed@gmail.com

City

-

Orcid

-

First Name

Atwa M.

Last Name

Hassan

MiddleName

-

Affiliation

Rift Valley Fever Vaccine Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt

Email

atwa_vet@yahoo.com

City

-

Orcid

-

First Name

Marwa

Last Name

Ahmed

MiddleName

-

Affiliation

Anaerobic bacteria department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt

Email

zidanmarwa@gmail.com

City

-

Orcid

-

First Name

Amal A.

Last Name

Mohamed

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB). Agricultural Research Center (ARC), Cairo, Egypt

Email

dr.amal1984@gmail.com

City

-

Orcid

-

First Name

El Helw H.

Last Name

Adel

MiddleName

-

Affiliation

Anaerobic Bacteria Vaccines Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt

Email

hamedelhelw@hotmail.com

City

-

Orcid

-

First Name

Darwish M.

Last Name

Darwish

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB). Agricultural Research Center (ARC), Cairo, Egypt

Email

darwishmahmoud2020@yahoo.com

City

-

Orcid

-

Volume

56

Article Issue

13

Related Issue

53224

Issue Date

2025-12-01

Receive Date

2024-05-04

Publish Date

2025-12-01

Page Start

21

Page End

28

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 56_Issue 13_Pages 21-28.pdf

PDF . 759.3kB

Link

https://ejvs.journals.ekb.eg/article_384787.html

Detail API

http://journals.ekb.eg?_action=service&article_code=384787

Order

384,787

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

A Recent Approach for In-vitro Evaluation of the Inactivated Rift Valley Fever Vaccine

Details

Type

Article

Created At

01 Feb 2025