The present study was conducted to evaluate the toxic effects resulted from acrylamide (ACR) and the protective effects of Moringa oleifera (M. oleifera) nanoparticles to adult male rats. Twenty adult male rats were randomly divided into four groups: Group I (Control group); Group II (Acrylamide group): rat received 50 mg/kg b.wt. of ACR in drinking water for 3 weeks; Group III (M. oleifera nanoparticles group): rats received 50 mg/kg b.wt of M. oleifera nanoparticles daily for 3 weeks; and Group IV (M. oleifera nanoparticles + Acrylamide group): rats were given 50 mg/kg b.wt of ACR and received M. oleifera nanoparticles 50 mg/kg b.wt orally, daily, at the same time for 3 weeks. Blood and tissue samples were collected for the physiological histological, and histochemical studies.

The histological study revealed that rats exposed to ACR had significant damage to their kidneys, livers, and testicles, along with inflammatory changes and structural deformities. Treatment with M. oleifera nanoparticles improve histopathological changes and reduced susceptibility to ACR toxicity in adult male rats. Three-week treatment of M. oleifera nanoparticles largely recover the reduction in proteins and carbohydrates observed in ACR administration. The application of M. oleifera nanoparticles with ACR significantly reduce the histochemical damage that caused by ACR. The treatment also reduce ACR's destructive impact on activities of ALT, AST enzymes, and MDA, creatinine, and urea levels while increase SOD enzyme activity. From the results one can suggest that M. oleifera nanoparticles is a potential cytoprotective agent against ACR's pathological toxicity.