Background:  Antimicrobial drug resistance has become a global health problem. There is a need for a newer, more efficient, and potent source of antibiotics against these drug-resistant pathogens, especially the Beta-lactamase drug-resistant organisms. This study examines the antibacterial activity of Streptomyces isolates on Beta-lactamase bacteria. Method: Soil Samples were collected from various sites within Mogadishu and screened for the isolation of Streptomyces following the conventional microbiological method. The soil samples were analyzed for their proximate content and were pretreated and inoculated onto a Czepadox agar modified with 50mg/mL cycloheximide and incubated for 5 – 7 days at 37^oc. Streptomyces-like isolates were purified and tested for various biochemical and sugar fermentation tests. The Antimicrobial activity of the Streptomyces isolates against Beta-lactamase organisms (Penicillin +Oxacillin resistant Staphylococcus aureus, Streptomycin resistant Shigella dysenterae, Oxacillin-resistant Pseudomonas aeruginosa, Carbapenem-resistant Salmonella typhi, Penicillin resistant Escherichia coli) was examined. The radical scavenging activity of Streptomyces isolates was examined. Results: Seventy-two (72) Streptomyces isolates were recovered, but only 10 isolates expressed high antibacterial activity against beta-lactamase-resistant bacteria. Streptomyces isolate GS 4 demonstrated the highest activity among the examined isolates. All the isolates had considerably high antioxidant activity. Conclusion: There was a high antibacterial activity of Streptomyces isolates recovered from the soil of Mogadishu against beta-lactamase-resistant bacteria. This study offers essential knowledge on soil properties and the potential of Streptomyces isolates for use in biotechnology and pharmaceutical industries and provides source material for bioactive antibiotics.