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High prevalence of multidrug-resistant extended spectrum beta lactamase -producing <i>Escherichia coli</i> in raw milk in Bangladesh

Article

Last updated: 01 Feb 2025

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Subjects

-

Tags

Antimicrobial resistance

Abstract

Background:  Raw milk is essential in our daily diet for food safety but poses a risk of contamination with harmful bacteria like ESβL Escherichia coli. Objectives: The study was undertaken to characterize multidrug-resistant E. coli and determine the antibiogram profile of the isolates recovered from raw milk. Methods: A total of 80 raw milk samples were collected from 20 milk selling points in Sylhet and analyzed for physio-chemical parameters, microbial loads, ESβL Escherichia coli, and their virulent factors with the association of AMR. Results: Results demonstrated that the mean specific gravity, fat%, protein%, lactose%, acidity%, SNF%, pH, and added water% were 1.026±0.0003, 3.83±0.072, 2.83±0.046, 4.08±0.080, 0.20±0.003, 7.47±0.144, 6.38±0.035, 1.72±0.034, respectively. The mean aerobic mesophilic count and mean coliform count were (2.72×10)7 CFU/ml and 1.53×(10)6 CFU/ml, respectively. We found the prevalence of E. coli was 60% (48/80) in raw milk, and PCR results revealed that all E. coli isolates were positive for the stx1 (6.25%), eaeA (6.25%), blaCITM (8.33%), blaSHV (6.25%), blaTEM (14.58%), and tetA (50%) genes. The antibiogram results demonstrated that all the isolates were most resistant to ampicillin and tetracycline (100%), amoxicillin (79.17%), ceftriaxone and ceftazidime (62.5%), streptomycin (58.53%) and gentamycin (60%), whereas vancomycin (79.17%), ciprofloxacin (75%) and meropenem (54.17%) were the most sensitive. Conclusion: Our study underscores the urgent need for stringent hygiene measures in milk production and emphasizes judicious antibiotic use in safeguarding public health.

DOI

10.21608/mid.2024.258318.1735

Keywords

Aerobic Count, ESBL, Antibiotic-resistant gene, Coliform count, Raw milk

Authors

First Name

Sohel

Last Name

Rana

MiddleName

-

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

drsohel.sau@gmail.com

City

-

Orcid

-

First Name

Fahmida

Last Name

Fahim

MiddleName

Jaahan

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

fahmidafahim05@gmail.com

City

-

Orcid

-

First Name

Rimi

Last Name

Das

MiddleName

-

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

drimi1936@gmail.com

City

-

Orcid

-

First Name

Kh

Last Name

Abdullah

MiddleName

Saad

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

saadabdullah230@gmail.com

City

-

Orcid

-

First Name

SM

Last Name

Islam

MiddleName

Sertaz

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

shertazislam@gmail.com

City

-

Orcid

-

First Name

Nabil

Last Name

Mahim

MiddleName

Jahan

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

njmahim@gmail.com

City

-

Orcid

-

First Name

Nadia

Last Name

Sultana

MiddleName

-

Affiliation

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Email

nadiasultanabge@gmail.com

City

-

Orcid

-

First Name

Md

Last Name

Uddin

MiddleName

Nazim

Affiliation

Department of Livestock Production and Management, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

uddinmn.alm@sau.ac.bd

City

-

Orcid

-

First Name

Md

Last Name

Islam

MiddleName

Rafiqul

Affiliation

Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh

Email

rafiq.vetmed@sau.ac.bd

City

-

Orcid

-

First Name

Shahed

Last Name

Ahmad

MiddleName

-

Affiliation

Institute of Livestock Science and Technology (ILST), Brahmanbarria, Bangladesh

Email

ahmadismail.82.bau@gmail.com

City

-

Orcid

-

First Name

Kazi

Last Name

Zinnah

MiddleName

-

Affiliation

Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh, Department of Animal and Fish Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Email

zinnah.afb@sau.ac.bd

City

-

Orcid

-

First Name

Monira

Last Name

Noor

MiddleName

-

Affiliation

Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences; Sylhet Agricultural University

Email

noorm.dpp@sau.ac.bd

City

-

Orcid

-

First Name

Ferdaus

Last Name

Hossain

MiddleName

Mohd Altaf

Affiliation

Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh, Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh

Email

ferdaus.dps@sau.ac.bd

City

Sylhet

Orcid

-

Volume

6

Article Issue

1

Related Issue

53078

Issue Date

2025-02-01

Receive Date

2023-12-26

Publish Date

2025-02-01

Page Start

267

Page End

277

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 6_Issue 1_Pages 267-277.pdf

PDF . 866.1kB

Link

https://mid.journals.ekb.eg/article_341728.html

Detail API

http://journals.ekb.eg?_action=service&article_code=341728

Order

26

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

High prevalence of multidrug-resistant extended spectrum beta lactamase -producing <i>Escherichia coli</i> in raw milk in Bangladesh

Details

Type

Article

Created At

01 Feb 2025