Using nanoparticles as a partial replacement for cement components has become an issue of rapidly growing importance in the last few years to promote sustainable concrete and to avoid the adverse effect of carbon dioxide and CO2 emissions from the cement production process on the environment. The current study investigates the efficiency of using Nano Clay particles as a partial replacement for cement. The practical study mainly focused on the flexural behavior of reinforced concrete beams made with replacement Nano-clay particles by a 6% ratio of cement matrix to achieve the same compressive strength of the corresponding traditional concrete mix. To achieve the experimental study, sixteen beams were designed, cast, and experimentally tested. The sixteen specimens were cast using two types of concrete mixture. Eight of them were cast using a concrete mix with a Nano-clay ratio of 6% as a replacement for cement, and the others were cast using traditional concrete mix without Nano-clay additive. The flexural behavior of the RC beams specimens concerning failure mode, and load-deflection behavior were presented and discussed. It can be concluded that the concrete enhanced with Nanoparticles gives better crack distribution and more ductile behavior than the traditional concrete during the different loading stages due to the enhancement of both, tensile and bond strengths produced by adding Nano-clay as a replacement for cement.