Context: Artificial intelligence (AI) has a significant role on our daily life, understanding the perspectives of nurses regarding application of AI with hemodialysis patients is necessary. Aim:  the study aimed to assess nurses' knowledge and attitude regarding application of artificial intelligence in hemodialysis unit, Benha university hospital.  Design: A descriptive research design was used. Subject: Convenience sample of 61 nurses who are working in hemodialysis unit were recruited from August first 2024 till October end 2024, the study utilized the following two tools:  Tool I: Self-administered Questionnaire to assess nurses' knowledge about application of artificial intelligence in hemodialysis unit. Tool II: Nurses' attitude assessment scale toward AI. Results: illustrated that 52.5% of the studied nurses had poor level of total knowledge about applying artificial intelligence in hemodialysis unit, 77% of them had positive attitude toward AI, as well as there was a statistically significant correlation between total knowledge of the studied nurses and their attitude toward AI. Conclusion: The result concluded deficiency in nurses' level of knowledge about applying artificial intelligence in hemodialysis unit and positive attitude also there was a positive correlation between total level of their knowledge and attitude toward AI.  Recommendation: Ongoing education and appropriate training of nurses for the new technology of artificial intelligence including the technical aspects and ethical consideration to acquire the necessary knowledge and skills to avoid medical errors