The trammel net shrimp fishery, located in Kotabaru, South Kalimantan, Indonesia, is a small-scale fishery that serves as a primary supplier to the food industry for international markets. PT Sekar Laut, Tbk, one of the companies relying on this fishery for raw materials, particularly yellow shrimp (Metapenaeus brevicornis) and banana shrimp (Penaeus merguensis), requires Marine Stewardship Council (MSC) certification to access global markets. A pre-assessment for MSC certification of this fishery revealed that limited spatial data, especially species-specific geographic information, hindered the ability to provide the necessary species distribution data for the fishery improvement project (FIP) in Kotabaru. This study aimed to explore spatial diversity in species composition and fishing coordinates collected from trammel net fisheries operating in the Kotabaru Waters from January to August 2024. The data will be used for fisheries management to support the yellow shrimp and banana shrimp FIP in Kotabaru. Using Shannon-Wiener's index (H') and Margalef's richness index (S), the spatial diversity across various fishing grounds was assessed, documenting significant variability in species composition and distribution. The results showed diversity indices ranging from 0 to 1.8 and richness indices from 1 to 15, indicating complex spatial patterns that suggest areas of both high conservation value and fishing pressure. The study also employed cluster analysis to identify patterns in fishing ground usage, which can inform more localized management strategies. These findings highlight the need for adaptive management approaches that consider spatial and temporal biodiversity fluctuations to enhance sustainability and support MSC certification.